Effect of Different Doses of Staurosporine For Neuronal Differentiation

Purpose: The present study was designed to investigate the effect of different doses of Staurosporine on differentiation of PC-12 cell line into differentiated neuronal cells. Materials and Methods: PC-12 cell line as a useful model system for neurobiological studies was maintained in RPMI 1640 supplemented with 10% FBS. Staurosporine were added to culture medium in different concentrations (110, 214, 316 and 1000nM), then morphology and apoptotic index were assessed by total neurite length estimation and TUNEL staining. Results: Data showed that total neurite length of PC-12 cells that were treated with 110, 214, 316 and 1000nM was 44±9.3, 153±15.0, 104±10.4 and 31±9.6μm respectively. Therefore PC-12 cells that were treated with 214nM Staurosporine had the greatest neurite length in compression with the other concentrations (P<0.05). In addition apoptotic index in these concentrations was 3±0.7,4±0.8,8±1.0 and 10±1.1 respectively, Therefore PC-12 cells that were treated with 110 and 214nM Staurosporine had minimal apoptotic index (P<0.05). Conclusion: Finally it can be concluded that 214nM concentration of Staurosporine is an optimum concentration for differentiation of neuronal cell lines to differentiated neuronal cells.